spfpХранение и переработка сельхозсырьяStorage and Processing of Farm Products2072-96692658-767XРОСБИОТЕХ10.36107/spfp.2020.376spfp-152Research ArticleИССЛЕДОВАНИЕ СВОЙСТВ ВЕЩЕСТВ И ПРОДУКЦИИ АПКRESEARCH ON TRAITS OF SUBSTANCES AND AGRIBUSINESS PRODUCTSОценка иммуногенности и содержания антигена А. ovis экспериментального образца адсорбированной инактивированной культурально-клеточной вакцины против анаплазмоза овецEvaluation of the Immunogenicity and Antigen Content of A. Ovis of an Experimental Sample of the Adsorbed Inactivated Culture-Cell Vaccine Against Sheep AnaplasmosisГлушаковР. В.GlushakovR. V.

Роман Витальевич Глушаков

115088, город Москва, 3-Й Угрешский проезд, дом 8 строение 9

Roman V. Glushakov

115088, Moscow, 3rd Ugreshsky proezd, house 8, building 9 

roman_nv@mail.ruВасиленкоИ. А.VasilenkoI. A.

Иван Александрович Василенко

115088, город Москва, 3-Й Угрешский проезд, дом 8 строение 9

Ivan A. Vasilenko

115088, Moscow, 3rd Ugreshsky proezd, house 8, building 9 

zhannadark@mail.ruДонскихА. А.DonskikhA. A.

Алексей Алексеевич Донских

115088, город Москва, 3-Й Угрешский проезд, дом 8 строение 9

Alexey A. Donskikh

115088, Moscow, 3rd Ugreshsky proezd, house 8, building 9 

alex.a.donskih@gmail.comЩетининМ. П.ShchetininM. P.

Михаил Павлович Щетинин

125371, город Москва, Волоколамское шоссе, 11

Mikhail P. Shchetinin

125371, Moscow, Volokolamskoe highway, 11 

shchetininmihail@mgupp.ruООО «ЕвроПак»РоссияLLC “EuroPack”Russian FederationООО «ЕвроПак»РоссияLLC „EuroPack“Russian FederationФГБОУ ВО «МГУПП»РоссияFSBEI HE “MGUPP”Russian Federation2020130520210382101Copyright © Глушаков Р.В., Василенко И.А., Донских А.А., Щетинин М.П., 20212021Глушаков Р.В., Василенко И.А., Донских А.А., Щетинин М.П.Glushakov R.V., Vasilenko I.A., Donskikh A.A., Shchetinin M.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.spfp-mgupp.ru/jour/article/view/152

Анаплазмоз овец – кровепаразитарная трансмиссивная болезнь, вызываемая эндоглобулярным внутриэритроцитарным паразитом Anaplasma. Основные признаки анаплазмоза – анемия, исхудание, потеря продуктивности и нарушение воспроизводительной функции животных. Перспективным для профилактики анаплазмоза овец является вакцинация их адсорбированной инактивированной культурально-клеточной вакциной. Проведенные исследования позволили оценить иммуногенность экспериментального образца вакцины, изготовленной с использованием препаратов смешанных культур лейкоцитов от иммунизированных животных, и содержание антигена А. ovis с помощью ПЦР. Сравнение экспериментальных образцов культуральноклеточной вакцины до и после хранения по показателям иммуногенности и содержанию антигена A. ovis с экстраполяцией степени спонтанной инактивации вакцины с реальной длительностью хранения сроком 1 год показало, что вышеуказанные показатели не ухудшились.

Sheep anaplasmosis is a blood-parasitic vector-borne disease caused by the endoglobular intra-erythrocyte parasite Anaplasma. The main signs of anaplasmosis are anemia, emaciation, loss of productivity and violation of the reproductive function of animals. Vaccination with an adsorbed inactivated culture-cell vaccine is promising for the prevention of sheep anaplasmosis. The conducted studies made it possible to evaluate the immunogenicity of an experimental sample of a vaccine made using preparations of mixed cultures of leukocytes from immunized animals, and the content of the A. ovis antigen using PCR. Comparison of experimental samples of culture-cell vaccine before and after storage in terms of immunogenicity and A. ovis antigen content with extrapolation of the degree of spontaneous inactivation of the vaccine with a real storage duration of 1 year showed that the above indicators did not deteriorate.

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The authors declare that there are no conflicts of interest present.