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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">spfp</journal-id><journal-title-group><journal-title xml:lang="ru">Хранение и переработка сельхозсырья</journal-title><trans-title-group xml:lang="en"><trans-title>Storage and Processing of Farm Products</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-9669</issn><issn pub-type="epub">2658-767X</issn><publisher><publisher-name>РОСБИОТЕХ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36107/spfp.2021.228</article-id><article-id custom-type="elpub" pub-id-type="custom">spfp-228</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИССЛЕДОВАНИЕ СВОЙСТВ ВЕЩЕСТВ И ПРОДУКЦИИ АПК</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>RESEARCH ON TRAITS OF SUBSTANCES AND AGRIBUSINESS PRODUCTS</subject></subj-group></article-categories><title-group><article-title>Разработка технологии извлечения комплекса биологически активных веществ из корневых культур in vitro лекарственных растений</article-title><trans-title-group xml:lang="en"><trans-title>Development of a Technology for Extraction of a complex of Biologically Active Substances from in Vitro Root Crops of Medicinal Plants</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Асякина</surname><given-names>Людмила Константиновна</given-names></name><name name-style="western" xml:lang="en"><surname>Asyakina</surname><given-names>Lyudmila K.</given-names></name></name-alternatives><email xlink:type="simple">alk_kem@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фотина</surname><given-names>Наталья Вячеславовна</given-names></name><name name-style="western" xml:lang="en"><surname>Fotina</surname><given-names>Natasha V.</given-names></name></name-alternatives><email xlink:type="simple">fotina.natashenka@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Степанова</surname><given-names>Анна Александровна</given-names></name><name name-style="western" xml:lang="en"><surname>Stepanova</surname><given-names>Anna A.</given-names></name></name-alternatives><email xlink:type="simple">annstepanova.04@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Еремеева</surname><given-names>Наталья Ивановна</given-names></name><name name-style="western" xml:lang="en"><surname>Eremeeva</surname><given-names>Natalia I.</given-names></name></name-alternatives><email xlink:type="simple">annstepanova.04@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Просеков</surname><given-names>Александр Юрьевич</given-names></name><name name-style="western" xml:lang="en"><surname>Prosekov</surname><given-names>Alexander Y.</given-names></name></name-alternatives><email xlink:type="simple">annstepanova.04@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО «Кемеровский Государственный университет»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Kemerovo State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff xml:lang="ru" id="aff-2"><institution>ФГБОУ ВО «Кемеровский Государственный университет»</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>07</day><month>08</month><year>2021</year></pub-date><volume>0</volume><issue>3</issue><fpage>95</fpage><lpage>104</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Асякина Л.К., Фотина Н.В., Степанова А.А., Еремеева Н.И., Просеков А.Ю., 2022</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="ru">Асякина Л.К., Фотина Н.В., Степанова А.А., Еремеева Н.И., Просеков А.Ю.</copyright-holder><copyright-holder xml:lang="en">Asyakina L.K., Fotina N.V., Stepanova A.A., Eremeeva N.I., Prosekov A.Y.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.spfp-mgupp.ru/jour/article/view/228">https://www.spfp-mgupp.ru/jour/article/view/228</self-uri><abstract><p>В работе представлены результаты научных исследований по разработке технологии извлечения сухих экстрактов из биомассы корневых культур in vitro клеток лекарственных растений Сибирского Федерального округа кодонопсиса мелковолосистого Codonopsis pilosula, любки двулистной Platanthera bifolia и лимонника китайского Schisandra chinensis. В качестве метода извлечения БАВ из растительных образцов был применен метод экстракции по Сокслету. Оптимизация выделения экстрактов осуществлялась по трем параметрам: температуре, продолжительности процесса и соотношению объема органического растворителя к массе растительного образца (гидромодуль), т.е. данные параметры выступали в качестве изменяемых. В ходе эксперимента удалось подобрать оптимальные параметры для выделения сухих экстрактов из каждого лекарственного растения. Для кодонопсиса мелковолосистого оптимальными параметрами являются: температура экстракции 40 °С, продолжительность процесса 60 мин и соотношение объема растворителя к количеству высушенной биомассы корневых культур клеток растений 1:10. Наиболее эффективным экстрагентом в отношении Codonopsis pilosula является этилацетат. Для корневых культур любки двулистной применяли значения следующих параметров: температура 40 °С, продолжительность экстрагирования 60 мин, гидромодуль 1:10. Экстракцию проводили ацетоном. Процесс экстрагирования БАВ из лимонника китайского осуществляли ацетоном при температуре процесса 40 °С в течение 60 мин в соотношении экстрагента к образцу 1:10. Использование в эксперименте значений данных параметров позволит добиться максимального выхода экстрактов БАВ из лекарственных растений при минимально возможных затратах на ресурсы (затраты на время, реактивы и др.).</p></abstract><trans-abstract xml:lang="en"><p>The paper presents the results of scientific research on the development of technology for the extraction of dry extracts from the biomass of root cultures in vitro of cells of medicinal plants of the Siberian Federal District of Codonopsis pilosula, Platanthera bifolia and Schisandra chinensis. The Soxhlet extraction method was used as a method for extracting biologically active substances from plant samples. The extraction of extracts was optimized according to three parameters: temperature, duration of the process, and the ratio of the volume of the organic solvent to the mass of the plant sample (hydromodule), i.e. these parameters were modifiable. During the experiment, it was possible to select the optimal parameters for the isolation of dry extracts from each medicinal plant. Optimum parameters for Codonopsis pilosula are: the extraction temperature is 40 ° C, the process duration is 60 min, and the ratio of the volume of the solvent to the amount of dried biomass of root cultures of plant cells is 1:10. The most effective extractant for Codonopsis pilosula is ethyl acetate. The following parameters were used for the root cultures of Platanthera bifolia: temperature 40 ° C, extraction time 60 min, hydromodule 1:10. Extraction was performed with acetone. The process of extracting biologically active substances from Schisandra chinensis was carried out with acetone at a process temperature of 40 ° C for 60 min in a ratio of the extractant to the sample of 1:10. The use of the values of these parameters in the experiment will make it possible to achieve the maximum yield of BAS extracts from medicinal plants at the lowest possible cost of resources (cost of time, reagents, etc.).</p></trans-abstract><kwd-group xml:lang="ru"><kwd>экстракция</kwd><kwd>лекарственные растения</kwd><kwd>БАВ</kwd><kwd>корневые культуры</kwd><kwd>параметры</kwd><kwd>органический растворитель</kwd></kwd-group><kwd-group xml:lang="en"><kwd>extraction</kwd><kwd>medicinal plants</kwd><kwd>biologically active substances</kwd><kwd>root cultures</kwd><kwd>parameters</kwd><kwd>organic solvent</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Babich O., Sukhikh S., Pungin A., Ivanova S., Asyakina L., Prosekov A. Modern Trends in the In Vitro Production and Use of Callus, Suspension Cells and Root Cultures of Medicinal Plants // Molecules. 2020. Vol. 25. 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