spfpХранение и переработка сельхозсырьяStorage and Processing of Farm Products2072-96692658-767XРОСБИОТЕХ10.36107/spfp.2022.356spfp-356Research ArticleБИОТЕХНОЛОГИЧЕСКИЕ И МИКРОБИОЛОГИЧЕСКИЕ АСПЕКТЫBIOTECHNOLOGICAL AND MICROBIOLOGICAL ASPECTSОценка потенциала пропионовокислых бактерий для получения постбиотиковEvaluation of the Potential of Propionic Acid Bacteria for Obtaining Postbioticshttps://orcid.org/0000-0002-5360-8955БегуноваАнна ВасильевнаBegunovaAnna V.

научный сотрудник Центральной лаборатории микробиологии, кандидат технических наук, SPIN-код автора: 1155-4050

a_begunova@vnimi.orghttps://orcid.org/0000-0002-1522-3798ЖижинНиколай АнатольевичZhizhinNikolay A.

научный сотрудник лаборатории технохимического контроля, кандидат технических наук

n_zhizhin@vnimi.orgФГАНУ "Всероссийский научно-исследовательский институт молочной промышленности"РоссияAll-Russian Dairy Research InstituteRussian Federation2022141020220485101Copyright © Бегунова А.В., Жижин Н.А., 20222022Бегунова А.В., Жижин Н.А.Begunova A.V., Zhizhin N.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.spfp-mgupp.ru/jour/article/view/356

Введение. Многочисленные современные исследования показали, что продукты метаболизма пробиотических культур, как и пробиотические микроорганизмы могут оказывать положительные эффекты на здоровье потребителя. Их использование является профилактической стратегией для укрепления здоровья человека. Цель. Охарактеризовать потенциал штамма P. shermanii Э2 для использования при получении постбиотиков. Материалы и методы. Штамм P. shermanii Э2 культивировали на питательной среде следующего состава: дрожжевой автолизат – 40 см3/дм3, KH2PO4 – 4 г/дм3, CoCl2 – 1 см3/дм3, гидролизованное молоко до 1 дм3. Ферментативную активность штамма определяли с использованием тест-системы API ZYM («BioMerieux», Франция). Протеолитическую активность определяли методом TNBS (2,4,6-тринитробензолсульфоновая кислота) и выражали в ммоль/л-эквивалентов лейцина. Антиоксидантную активность определи методом ORAC. Оценку содержания органических кислот проводили методом высокоэффективной жидкостной хроматографии (ВЭЖХ), а определение витамина В12 методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (ВЭЖХ-МС). Результаты. Определен профиль ферментативной активности P. shermanii Э2, протеолитическая и антиоксидантная активность, кроме того установлено содержание органических кислот и витамина В12 в бесклеточных супернатантах. Установлено, что штамм обладает выраженной аминопептидазной активностью, высокой активностью кислой фосфатазы, α-галактозидазы и β-галактозидазы. Однако активностей трипсина, липазы, β-глюкорнидазы, β-глюкозидазы, N-ацетил-β-глюкозаминидазы, α-маннозидазы и α-фруктозидазы не наблюдалось. Показано повышение протеолитической и антиоксидантной активности в процессе культивирования P. shermanii Э2. Наибольших значений протеолитическая и антиоксидантная активности достигли через 72 ч культивирования P. shermanii Э2. Кроме того, в бесклеточных супернатантах, полученных через 72 ч культивирования P. shermanii Э2 показано наибольшее содержание пропионовой, уксусной и янтарной кислот – (4858,0±173) мг/дм3, (1542,0±44) мг/дм3, (338,0±11) мг/дм3 соответственно, а количество витамина В12 составило (3,67±0,05) мкг/дм3. Учитывая, что пробиотические свойства штаммов связаны с образованием определенных метаболитов, проведенные исследования позволяют сделать вывод о пробиотическом потенциале штамма P. shermanii Э2 и возможности его использования не только в составе заквасок, но и при получении постбиотиков. Выводы. Применение постбиотиков при производстве продуктов питания в качестве функциональных ингредиентов будет способствовать расширению рынка функциональных продуктов, а определение их биологической активности позволит расширить область применения постбиотиков.

Introduction

Introduction. Numerous modern studies show that the products of potentially probiotic cultures, like probiotic microorganisms, can cause positive effects on the health of users. Their using is a preventive strategy for approaching human health.

Purpose

Purpose. To characterize the potential of the P. shermani E2 strain for use for the production of postbiotics.

Materials and methods

Materials and methods. The P. shermanii E2 strain was cultivated on a nutrient medium with the following composition: yeast autolysate, 40 cm3/dm3, KH2PO4, 4 g/dm3, CoCl2, 1 cm3/dm3, and hydrolyzed milk up to 1 dm3. The enzymatic activity of the strain was determined by using the API ZYM test system (BioMerieux, France). Proteolytic activity was determined by the TNBS method (2,4,6-trinitrobenzenesulfonic acid) and expressed in mmol/l leucine equivalents. Antioxidant activity was determined by the ORAC method. The content of organic acids was evaluated by high performance liquid chromatography (HPLC), and vitamin B12 was determined by high performance liquid chromatography with a mass spectrometric detector (HPLC-MS).

Results

Results. The profile of the enzymatic activity of P. shermani E2, it’s proteolytic and antioxidant activity was determined, in addition, the content of organic acids and vitamin B12 in cell-free supernatants was determined. It was found that the strain has a pronounced aminopeptidase activity, high activity of acid phosphatase, α-galactosidase and β-galactosidase. However, the activities of trypsin, lipase, β-glucornidase, β-glucosidase, N-acetyl-β-glucosaminidase, α-mannosidase and α-fructosidase were not observed. An increase in proteolytic and antioxidant activity during the cultivation of P. shermanii E2 was shown. An increase in proteolytic and antioxidant activity during the cultivation of P. shermanii E2 was shown. The highest values of proteolytic and antioxidant activity were reached after 72 h of cultivation P. shermanii E2. In addition, cell-free supernatants obtained after 72 hours of P. shermanii E2 cultivation showed the highest content of propionic, acetic and succinic acids – (4858.0±173) mg/dm3, (1542.0±44) mg/dm3, (338.0±11) mg/dm3, respectively, and the amount of vitamin B12 was (3.67±0.05) µg/dm3. Taking into account that the probiotic properties of the strains are associated with the formation of certain metabolites, the conducted studies allow us to conclude that the P. shermani E2 strain has a probiotic potential and the possibility of its use not only as part of starter cultures, but also in the production of postbiotics.

Conclusions

Conclusions. The use of postbiotic in food production as food additives will correspond to the expansion of the market for functional products, and the determination of their biological applicability will expand the field of postbiotics.

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The authors declare that there are no conflicts of interest present.