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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">spfp</journal-id><journal-title-group><journal-title xml:lang="ru">Хранение и переработка сельхозсырья</journal-title><trans-title-group xml:lang="en"><trans-title>Storage and Processing of Farm Products</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-9669</issn><issn pub-type="epub">2658-767X</issn><publisher><publisher-name>РОСБИОТЕХ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.36107/spfp.2025.1.621</article-id><article-id custom-type="elpub" pub-id-type="custom">spfp-621</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>СЫРЬЕ И ДОБАВКИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>RAW MATERIALS AND ADDITIVES</subject></subj-group></article-categories><title-group><article-title>Пробиотическая кормовая добавка с микробным бета-каротином: разработка и свойства</article-title><trans-title-group xml:lang="en"><trans-title>Probiotic Feed Additive with Microbial Beta-Carotene: Development and Properties</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5220-7877</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ядерец</surname><given-names>Вера Владимировна</given-names></name><name name-style="western" xml:lang="en"><surname>Yaderets</surname><given-names>Vera V.</given-names></name></name-alternatives><email xlink:type="simple">verayaderetz@yandex.ry</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6652-4136</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Карпова</surname><given-names>Наталья Викторовна</given-names></name><name name-style="western" xml:lang="en"><surname>Karpova</surname><given-names>Natalia V.</given-names></name></name-alternatives><email xlink:type="simple">ashatanr@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3894-0255</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Глаголева</surname><given-names>Елена Викторовна</given-names></name><name name-style="western" xml:lang="en"><surname>Glagoleva</surname><given-names>Elena V.</given-names></name></name-alternatives><email xlink:type="simple">glagolevaev@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5161-5051</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Джавахия</surname><given-names>Вахтанг Витальевич</given-names></name><name name-style="western" xml:lang="en"><surname>Dzhavakhiya</surname><given-names>Vakhtang V.</given-names></name></name-alternatives><email xlink:type="simple">vahoru@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Российский биотехнологический университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Russian Biotechnological University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>17</day><month>04</month><year>2025</year></pub-date><volume>33</volume><issue>1</issue><fpage>141</fpage><lpage>160</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ядерец В.В., Карпова Н.В., Глаголева Е.В., Джавахия В.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Ядерец В.В., Карпова Н.В., Глаголева Е.В., Джавахия В.В.</copyright-holder><copyright-holder xml:lang="en">Yaderets V.V., Karpova N.V., Glagoleva E.V., Dzhavakhiya V.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.spfp-mgupp.ru/jour/article/view/621">https://www.spfp-mgupp.ru/jour/article/view/621</self-uri><abstract><sec><title>Введение</title><p>Введение: Комбинированные кормовые добавки, сочетающие пробиотические микроорганизмы и β-каротин, обладают потенциалом для повышения эффективности питания и укрепления здоровья сельскохозяйственных животных. Однако такие средства отсутствуют среди зарегистрированных препаратов, а микробные источники β-каротина используются крайне ограниченно. Это обусловливает необходимость поиска новых биотехнологических решений в области кормопроизводства.</p></sec><sec><title>Цель</title><p>Цель: Разработка каротинсодержащей пробиотической кормовой добавки (КД) и оценка её безопасности и эффективности при включении в рационы бройлерных цыплят и свиней на откорме.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы: Экспериментальный образец КД включал спорообразующие бактерии Bacillus subtilis BKM B–3826D и Bacillus licheniformis BKM B–3825D (по 5×10⁷ КОЕ/г каждого штамма) и инактивированную биомассу Mycolicibacterium neoaurum BKM Ac–3067D (не менее 250 мкг β-каротина/г). Токсикологическая оценка проводилась в соответствии с ГОСТ 32296–2013, класс токсичности определён по ГОСТ 12.1.007–76. Биологическую активность изучали на бройлерах и поросятах стандартными методами.</p></sec><sec><title>Результаты</title><p>Результаты: Разработанная пробиотическая добавка отнесена к 4 классу опасности (малотоксичная). При добавлении КД в рацион бройлеров (1,0 кг/т) среднесуточный прирост массы увеличился на 4,3%, а содержание витамина А в печени — в 1,4 раза. У поросят прирост живой массы составил 6,7% по сравнению с контролем.</p></sec><sec><title>Выводы</title><p>Выводы: Установлены безопасность и биологическая эффективность новой пробиотической кормовой добавки с микробным β-каротином. Каротиносодержащая пробиотическая добавка может быть рекомендована к использованию в специализированных и фермерских хозяйствах.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Introduction</title><p>Introduction: Combined feed additives that incorporate probiotic microorganisms and β-carotene have the potential to enhance the nutritional efficiency and health status of farm animals. However, such formulations are not currently represented among registered feed products, and microbial sources of β-carotene are used only to a limited extent. This highlights the need for novel biotechnological solutions in feed production.</p></sec><sec><title>Purpose</title><p>Purpose: To develop a carotene-containing probiotic feed additive (FA) and to evaluate its safety and effectiveness when included in the diets of broiler chickens and fattening pigs.</p></sec><sec><title>Materials and Methods</title><p>Materials and Methods: The experimental FA sample contained spore-forming bacteria Bacillus subtilis BKM B–3826D and Bacillus licheniformis BKM B–3825D (5×10⁷ CFU/g of each strain), along with inactivated biomass of Mycolicibacterium neoaurum BKM Ac–3067D (containing at least 250 µg of β-carotene per gram). Toxicological evaluation was performed in accordance with GOST 32296–2013, and toxicity class was determined according to GOST 12.1.007–76. Biological activity was assessed using standard methods in broiler chickens and piglets.</p></sec><sec><title>Results</title><p>Results: The developed probiotic feed additive was classified as hazard class 4 (low toxicity). When included in broiler diets at 1.0 kg/ton, it resulted in a 4.3% increase in average daily weight gain and a 1.4-fold increase in liver vitamin A content. In piglets, live weight gain increased by 6.7% compared to the control group.</p></sec><sec><title>Conclusion</title><p>Conclusion: The safety and biological effectiveness of the new carotene-containing probiotic feed additive with microbial β-carotene were confirmed. This additive may be recommended for use in specialized and small-scale farming operations.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>пробиотическая кормовая добавка</kwd><kwd>β-каротин микробного происхождения</kwd><kwd>Bacillus subtilis</kwd><kwd>Mycolicibacterium neoaurum</kwd><kwd>токсикологическая оценка кормовой добавки</kwd><kwd>продуктивность животных</kwd></kwd-group><kwd-group xml:lang="en"><kwd>probiotic feed additive</kwd><kwd>microbial β-carotene</kwd><kwd>Bacillus subtilis</kwd><kwd>Mycolicibacterium neoaurum</kwd><kwd>toxicological evaluation of the feed additive</kwd><kwd>animal productivity</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена в рамках государственного задания Министерства науки и высшего образования Российской Федерации (тема № 123012000071-1).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Буяров, В. С., Червонова, И. В., Меднова, В. В., &amp; Ильичева, И. Н. (2020). Эффективность применения фитобиотиков в птицеводстве (обзор). Вестник аграрной науки, 3(84), 44-59. https://doi.org/10.17238/issn2587-666X.2020.3.44</mixed-citation><mixed-citation xml:lang="en">Buyarov, V.S., Chervonova, I.V., Mednova, V.V., &amp; Ilyicheva, I.N. (2020). Efficiency of application of phytobiotics in poultry farming (Rewiew). Bulletin of Agrarian science, 3(84), 44-59. (in Russ.) https://doi:10.17238/issn2587-666X.2020.3.44</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Злепкин В. А., Злепкин Д. А., Рудаков А. В., &amp; Злепкина Н. А. (2020). Влияние бета-каротинсодержащего препарата совместно с пробиотиками на переваримость и использование питательных веществ рационов цыплятами-бройлерами. Птицеводство, 7-8, 34-38.</mixed-citation><mixed-citation xml:lang="en">Ivanova, N.V., &amp; Radjabov, R.G. (2019). Resource-saving technologies in pig production. Bulletin of Don State Agrarian University, 3-1, 5-9. (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">https://doi.org/10.33845/0033-3239-2020-69-7-8-34-38</mixed-citation><mixed-citation xml:lang="en">Mailyan, E. S. (2021) The problem of the use of antibiotics in animal husbandry and ways to control microbial antibiotic resistance. BIO, 12(255), 4-16 (In Russ.)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Иванова, Н. В., &amp; Раджабов, Р. Г. (2019). Ресурсосберегающие технологии в свиноводстве. Вестник Донского государственного аграрного университета, 3-1, 5-9</mixed-citation><mixed-citation xml:lang="en">Yaderetz, V.V., Karpova, N.V., Glagoleva, E.V., Dzhavakhiya, V.V., Kartashov, M.I., Lenkova, T.N., &amp; Egorova T.A. (2024) The effect of a carotene containing probiotic feed additive on the productive performance in broilers. Ptitsevodstvo, 73(6), 19-24. (In Russ.) https://doi:10.33845/0033-3239-2024-73-6-19-24</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Маилян, Э. С. (2021). Проблема использования антибиотиков в животноводстве и пути контроля микробной антибиотикорезистентности. БИО, 12(255), 4-16.</mixed-citation><mixed-citation xml:lang="en">Yaderetz, V.V., Karpova, N.V., Dzhavakhiya, V.V., Glagoleva, E.V., &amp; Ostrenko, K.S. (2024) Investigation of the effectiveness of a carotene-containing probiotic supplement in feeding growing fattened pigs. Pigbreeding, 7, 45 – 48 (In Russ.) https://doi:10/37925/0039-713X-2024-7-45-48</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Морозова, Е. С., &amp; Мурленков Н. В. (2021). Эффективность влияния биопрепаратов на основе бактерий рода Bacillus в технологии выращивания поросят-отъемышей. Биология в сельском хозяйстве, 1(30), 21-24.</mixed-citation><mixed-citation xml:lang="en">Yaderetz, V.V., Karpova, N.V., Glagoleva, E.V., Dzhavakhiya, V.V., &amp; Ostrenko K.S. (2024) The influence of feed protein-carotene probiotic additive on hemato-biochemical parameters and intestinal biocenosis of fattening piglet. Veterinary medicine, 12, 44 – 48. (In Russ.) https://doi:10.30896/0042-4846.2024.27.12.44-48</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Овчарова, А. Н. &amp; Петраков Е. С. (2018). Физиологические показатели и продуктивность цыплят-бройлеров при использовании пробиотического препарата на основе бацилл . Проблемы биологии продуктивных животных, 1, 94-101. https://doi.org/10.25687/1996-6733.prodanimbiol.2018.1.94-101</mixed-citation><mixed-citation xml:lang="en">Alagawany, M., Abd El-Hack, M.E., Farag, M.R., Sachan, S., Karthik, K., &amp; Dhama, K. (2018) The use of probiotics as eco-friendly alternatives for antibiotics in poultry nutrition. Environ Sci Pollut Res, 25, 10611–8. https://doi:10.1007/s11356-018-1687-x</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Токарев, И. Н., &amp; А. В. Близнецов. (2017) Влияние пробиотической добавки Ветоспорин на интенсивность роста, конверсию корма и гематологические показатели поросят-отъёмышей. Российский электронный научный журнал, 1(23), 23-31.</mixed-citation><mixed-citation xml:lang="en">Al-Seraih, A.A., Alsereah, B.A., Alwaely, W.A., Al-Hejaj, M.Y. (2022) Effect of Bacillus subtilis as a Probiotic on the Productive and Physiological Performance of Broilers. Arch Razi Inst., 77(5), 1647-1653. https://doi:10.22092/ARI.2022.357803.2100.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Ядерец, В.В., Карпова, Н.В., Глаголева, Е.В., Джавахия, В.В., Карташов, М.И., Ленкова, Т.Н., &amp; Егорова, Т.А. (2024a). Влияние каротинсодержащей пробиотической добавки на продуктивные показатели бройлеров. Птицеводство, 6, 19-24. https://doi.org/10.33845/0033-3239-2024-73-6-19-24</mixed-citation><mixed-citation xml:lang="en">Awad, W.A., Ghareeb., K., Abdel-Raheem, S., Bohm, J. (2009) Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult Sci., 88(1), 49–56. https://doi: 10.3382/ps.2008-00244</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Ядерец, В.В., Карпова, Н.В., Джавахия, В.В., Глаголева, Е.В., &amp; Остренко, К.С. (2024b). Белково-каротиновая пробиотическая добавка в рационе свиней на откорме. Свиноводство, 7, 45-48. https://doi.org/10/37925/0039-713X-2024-7-45-48</mixed-citation><mixed-citation xml:lang="en">Azad, M.A.K., Sarker, M., Li, T., &amp; Yin, J. (2018) Probiotic species in the modulation of gut mmicrobiota: an overview. Biomed Res Int., 8, 9478630. https://doi:10.1155/2018/9478630</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Ядерец, В.В., Карпова, Н.В., Глаголева, Е.В., Джавахия, В.В., &amp; Остренко, К.С. (2024c). Влияние кормовой белково-каротиновой пробиотической добавки на гемато-биохимические показатели и кишечный биоценоз поросят на откорме. Ветеринария, 12, 44 – 48. https://doi.org/10.30896/0042-4846.2024.27.12.44-48</mixed-citation><mixed-citation xml:lang="en">Ban, Y., &amp; Guan, L.L. (2021) Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health. J Anim Sci Biotechnol, 12(1), 109. https://doi:10.1186/s40104-021-00630-x</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Alagawany, M., Abd El-Hack, M.E., Farag, M.R., Sachan, S., Karthik, K., &amp; Dhama, K. (2018). The use of probiotics as eco-friendly alternatives for antibiotics in poultry nutrition. Environmental Science and Pollution Research, 25, 10611–8. https://doi.org/10.1007/s11356-018-1687-x</mixed-citation><mixed-citation xml:lang="en">Bernardeau, M., Lehtinen, M.J. Forssten, S.D., &amp; Nurminen, P. (2017) Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality. J Food Sci Technol., 54(8), 2570-2584. https://doi:10.1007/s13197-017-2688-3</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Al-Seraih, A.A., Alsereah, B.A., Alwaely, W.A., Al-Hejaj, M.Y. (2022). Effect of Bacillus subtilis as a Probiotic on the productive and physiological performance of broilers. Archives of Razi Institute., 77(5), 1647-1653. https://doi.org/10.22092/ARI.2022.357803.2100.</mixed-citation><mixed-citation xml:lang="en">Grant, A., Gay, C.G., &amp; Lillehoj H.S. (2018) Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance growth, immunity, and gut health in poultry. Avian Pathol., 47(4), 339-351. https://doi:10.1080/03079457.2018.1464117</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Awad, W.A., Ghareeb., K., Abdel-Raheem, S., Bohm, J. (2009). Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poultry Science, 88(1), 49–56. https://doi.org/10.3382/ps.2008-00244</mixed-citation><mixed-citation xml:lang="en">Jha, R., Fouhse, J.M., Tiwari, U.P., Li, L., &amp; Willing, B.P. (2019) Dietary Fiber and Intestinal Health of Monogastric Animals. Front. Vet. Sci., 6, 48. https://doi:10.3389/fvets.2019.00048</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Azad, M.A.K., Sarker, M., Li, T., &amp; Yin, J. (2018). Probiotic species in the modulation of gut mmicrobiota: An overview. BioMed Research International, 8, 9478630. https://doi.org/10.1155/2018/9478630</mixed-citation><mixed-citation xml:lang="en">Jha, R., Das R, Oak, S., &amp; Mishra, P. (2020) Probiotics (Direct-Fed Microbials) in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health: a systematic review. Animals (Basel), 10(10), 1863. https://doi:10.3390/ani10101863</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Ban, Y., &amp; Guan, L.L. (2021). Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health. Journal of Animal Science and Biotechnology, 12(1), 109. https://doi.org/10.1186/s40104-021-00630-x</mixed-citation><mixed-citation xml:lang="en">Gadde, U.D., Oh, S., Lee, Y., Davis, E., Zimmerman, N., Rehberger, T., &amp; Lillehoj, H.S. (2017) Dietary Bacillus subtilis- based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens. Res. Vet. Sci., 114, 236–243. https://doi:10.1016/j.rvsc.2017.05.004</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Bernardeau, M., Lehtinen, M.J. Forssten, S.D., &amp; Nurminen, P. (2017). Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality. Journal of Food Science and Technology, 54(8), 2570-2584. https://doi.org/10.1007/s13197-017-2688-3</mixed-citation><mixed-citation xml:lang="en">Grant, A., Gay, C.G., &amp; Lillehoj, H.S. (2018) Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance growth, immunity, and gut health in poultry. Avian Pathol., 47(4), 339-351. https://doi:10.1080/03079457.2018.1464117</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Grant, A., Gay, C.G., &amp; Lillehoj, H.S. (2018). Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance growth, immunity, and gut health in poultry. Avian Pathology, 47(4), 339-351. https://doi.org/10.1080/03079457.2018.1464117</mixed-citation><mixed-citation xml:lang="en">Khalid, F., Khalid, A., Fu, Y., Hu, Q., Zheng, Y., Khan, S., &amp; Wang Z. (2021) Potential of Bacillus velezensis as a probiotic in animal feed: a review. J Microbiol., 59(7), 627-633. https://doi:10.1007/s12275-021-1161-1</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Jha, R., Fouhse, J.M., Tiwari, U.P., Li, L., &amp; Willing, B.P. (2019). Dietary fiber and intestinal health of monogastric animals. Frontiers in Veterinary Science, 6, 48. https://doi.org/10.3389/fvets.2019.00048</mixed-citation><mixed-citation xml:lang="en">Lewton, J.R,, Woodward, A.D., Moser, R.L., Thelen, K.M., Moeser, A.J., Trottier, N.L., Tempelman, R.J., &amp; Rozeboom, D.W. (2022) Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on immunity markers and intestinal morphology in diets fed to weanling pigs. Transl Anim Sci., 6(3), txac083. https://doi:10.1093/tas/txac083</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Jha, R., Das R, Oak, S., &amp; Mishra, P. (2020). Probiotics (Direct-Fed Microbials) in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health: A systematic review. Animals, 10(10), 1863. https://doi.org/10.3390/ani10101863</mixed-citation><mixed-citation xml:lang="en">Ma, T., Suzuki, Y., &amp; Guan, L.L. (2018) Dissect the mode of action of probiotics in affecting host-microbial interactions and immunity in food producing animals. Vet Immunol Immunopathol, 205, 35–48. https://doi:10.1016/j.vetimm.2018.10.004</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Gadde, U.D., Oh, S., Lee, Y., Davis, E., Zimmerman, N., Rehberger, T., &amp; Lillehoj, H.S. (2017). Dietary Bacillus subtilis- based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens. Research in Veterinary Science, 114, 236–243. https://doi.org/10.1016/j.rvsc.2017.05.004</mixed-citation><mixed-citation xml:lang="en">Meléndez-Martínez, A. J. (2019) An Overview of carotenoids, apocarotenoids, and vitamin A in agro-food, nutrition, health, and disease. Mol Nutr Food Res., 63, e1801045. https://doi:10.1002/mnfr.201801045</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Grant, A., Gay, C.G., &amp; Lillehoj, H.S. (2018) Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance growth, immunity, and gut health in poultry. Avian Pathology, 47(4), 339-351. https://doi.org/10.1080/03079457.2018.1464117</mixed-citation><mixed-citation xml:lang="en">Pandey, S., Doo, H., Keum, G.B., Kim, E.S., Kwak, J., Ryu, S., Choi, Y., Kang, J., Kim, S., Lee, N.R., Oh, K.K., Lee, J.H., &amp; Kim, H.B. Antibiotic resistance in livestock, environment and humans: One Health perspective. (2024) J Anim Sci Technol., 66(2), 266-278. https://doi:10.5187/jast.2023.e129</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Khalid, F., Khalid, A., Fu, Y., Hu, Q., Zheng, Y., Khan, S., &amp; Wang, Z. (2021) Potential of Bacillus velezensis as a probiotic in animal feed: a review. J Microbiol., 59(7), 627-633. https://doi.org/10.1007/s12275-021-1161-1</mixed-citation><mixed-citation xml:lang="en">Pluske, J. R., Turpin, D.L., &amp; Kim J.C. (2018) Gastrointestinal tract (gut) health in the young pig. Anim Nutr., 4(2), 187-196. https://doi:10.1016/j.aninu.2017.12.004</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Lewton, J.R,, Woodward, A.D., Moser, R.L., Thelen, K.M., Moeser, A.J., Trottier, N.L., Tempelman, R.J., &amp; Rozeboom, D.W. (2022). Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on immunity markers and intestinal morphology in diets fed to weanling pigs. Translational Animal Science, 6(3), txac083. https://doi.org/10.1093/tas/txac083</mixed-citation><mixed-citation xml:lang="en">Ramirez-Garzon, O., Barber, D., Meneses, L., &amp; Soust, M. (2024) Effect of gestational direct-fed microbials supplementation on the metabolic profile in periparturient dairy cows. Animals (Basel)., 14(20), 2928. https://doi:10.3390/ani14202928</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Ma, T., Suzuki, Y., &amp; Guan, L.L. (2018). Dissect the mode of action of probiotics in affecting host-microbial interactions and immunity in food producing animals. Veterinary Immunology and Immunopathology, 205, 35–48. https://doi.org/10.1016/j.vetimm.2018.10.004</mixed-citation><mixed-citation xml:lang="en">Ruiz Sella, S.R.B.R., Bueno, T., de Oliveira, A.A.B., Karp, S.G., &amp; Soccol, C.R. (2021) Bacillus subtilis natto as a potential probiotic in animal nutrition. Crit. Rev. Biotechnol. 2021, 41, 355–369. https://doi:10.1080/07388551.2020.1858019</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Meléndez-Martínez, A. J. (2019) An Overview of carotenoids, apocarotenoids, and vitamin A in agro-food, nutrition, health, and disease. Molecular Nutrition &amp; Food Research, 63, e1801045. https://doi.org/10.1002/mnfr.201801045</mixed-citation><mixed-citation xml:lang="en">Sarsour, A.H., Koltes, D.A., Kim, E.J., Persia, &amp; M.E. (2022) Effects of a direct fed microbial (DFM) on broiler chickens exposed to acute and chronic cyclic heat stress in two consecutive experiments. Poult Sci. 101(4), 101705. https://doi:10.1016/j.psj.2022.101705</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">Pandey, S., Doo, H., Keum, G.B., Kim, E.S., Kwak, J., Ryu, S., Choi, Y., Kang, J., Kim, S., Lee, N.R., Oh, K.K., Lee, J.H., &amp; Kim, H.B. (2024). Antibiotic resistance in livestock, environment and humans: One Health perspective. Journal of Animal Science and Technology, 66(2), 266-278. https://doi.org/10.5187/jast.2023.e129</mixed-citation><mixed-citation xml:lang="en">Shastak, Y., &amp; Pelletier, W. (2023) Review: Vitamin A supply in swine production: Current science and practical considerations. Applied Animal Science, 39(5), 289-305. https://doi:10.15232/aas.2023-02409</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Pluske, J. R., Turpin, D.L., &amp; Kim J.C. (2018). Gastrointestinal tract (gut) health in the young pig. Animal Nutrition, 4(2), 187-196. https://doi.org/10.1016/j.aninu.2017.12.004</mixed-citation><mixed-citation xml:lang="en">Telhig, S., Ben Said, L., Zirah, S., Fliss, I., &amp; Rebuffat, S. (2020) Bacteriocins to thwart bacterial resistance in gram negative bacteria. Front Microbiol., 11, 586433. https://doi:10.3389/fmicb.2020.586433</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Ramirez-Garzon, O., Barber, D., Meneses, L., &amp; Soust, M. (2024) Effect of gestational direct-fed microbials supplementation on the metabolic profile in periparturient dairy cows. Animals, 14(20), 2928. https://doi.org/10.3390/ani14202928</mixed-citation><mixed-citation xml:lang="en">Yaderets, V., Karpovam, N., Glagoleva, Е., Shibaeva, А., &amp; Dzhavakhiya, V. (2023) Bacillus subtilis RBT-7/32 and Bacillus licheniformis RBT-11/17 as new promising strains for use in probiotic feed additives. Microorganisms, 11(11), 2729. https://doi:10.3390/microorganisms11112729</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Ruiz Sella, S.R.B.R., Bueno, T., de Oliveira, A.A.B., Karp, S.G., &amp; Soccol, C.R. (2021). Bacillus subtilis natto as a potential probiotic in animal nutrition. Critical Reviews in Biotechnology, 41, 355–369. https://doi.org/10.1080/07388551.2020.1858019</mixed-citation><mixed-citation xml:lang="en">Yaderets, V., Karpova, N., Glagoleva, Е., Shibaeva, А., &amp; Dzhavakhiya, V. (2023) Enhanced β-Carotene production in Mycolicibacterium neoaurum Ac-501/22 by combining mutagenesis, strain selection, and subsequent fermentation optimization. Fermentation, 9(12), 1007. https://doi: /10.3390/fermentation9121007</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Sarsour, A.H., Koltes, D.A., Kim, E.J., Persia, &amp; M.E. (2022). Effects of a direct fed microbial (DFM) on broiler chickens exposed to acute and chronic cyclic heat stress in two consecutive experiments. Poultry Science Journal., 101(4), 101705. https://doi.org/10.1016/j.psj.2022.101705</mixed-citation><mixed-citation xml:lang="en">Sarsour, A.H., Koltes, D.A., Kim, E.J., Persia, &amp; M.E. (2022). Effects of a direct fed microbial (DFM) on broiler chickens exposed to acute and chronic cyclic heat stress in two consecutive experiments. Poultry Science Journal., 101(4), 101705. https://doi.org/10.1016/j.psj.2022.101705</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Shastak, Y., &amp; Pelletier, W. (2023). Review: Vitamin A supply in swine production: Current science and practical considerations. Applied Animal Science, 39(5), 289-305. https://doi.org/10.15232/aas.2023-02409</mixed-citation><mixed-citation xml:lang="en">Shastak, Y., &amp; Pelletier, W. (2023). Review: Vitamin A supply in swine production: Current science and practical considerations. Applied Animal Science, 39(5), 289-305. https://doi.org/10.15232/aas.2023-02409</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Telhig, S., Ben Said, L., Zirah, S., Fliss, I., &amp; Rebuffat, S. (2020). Bacteriocins to thwart bacterial resistance in gram negative bacteria. Frontiers in Microbiology, 11, 586433. https://doi.org/10.3389/fmicb.2020.586433</mixed-citation><mixed-citation xml:lang="en">Telhig, S., Ben Said, L., Zirah, S., Fliss, I., &amp; Rebuffat, S. (2020). Bacteriocins to thwart bacterial resistance in gram negative bacteria. Frontiers in Microbiology, 11, 586433. https://doi.org/10.3389/fmicb.2020.586433</mixed-citation></citation-alternatives></ref><ref id="cit34"><label>34</label><citation-alternatives><mixed-citation xml:lang="ru">Yaderets, V., Karpovam, N., Glagoleva, Е., Shibaeva, А., &amp; Dzhavakhiya, V. (2023). Bacillus subtilis RBT-7/32 and Bacillus licheniformis RBT-11/17 as new promising strains for use in probiotic feed additives. Microorganisms, 11(11), 2729. https://doi.org/10.3390/microorganisms11112729</mixed-citation><mixed-citation xml:lang="en">Yaderets, V., Karpovam, N., Glagoleva, Е., Shibaeva, А., &amp; Dzhavakhiya, V. (2023). Bacillus subtilis RBT-7/32 and Bacillus licheniformis RBT-11/17 as new promising strains for use in probiotic feed additives. Microorganisms, 11(11), 2729. https://doi.org/10.3390/microorganisms11112729</mixed-citation></citation-alternatives></ref><ref id="cit35"><label>35</label><citation-alternatives><mixed-citation xml:lang="ru">Yaderets, V., Karpova, N., Glagoleva, Е., Shibaeva, А., &amp; Dzhavakhiya, V. (2023). Enhanced β-Carotene production in Mycolicibacterium neoaurum Ac-501/22 by combining mutagenesis, strain selection, and subsequent fermentation optimization. Fermentation, 9(12), 1007. https://doi.org//10.3390/fermentation9121007</mixed-citation><mixed-citation xml:lang="en">Yaderets, V., Karpova, N., Glagoleva, Е., Shibaeva, А., &amp; Dzhavakhiya, V. (2023). Enhanced β-Carotene production in Mycolicibacterium neoaurum Ac-501/22 by combining mutagenesis, strain selection, and subsequent fermentation optimization. Fermentation, 9(12), 1007. https://doi.org//10.3390/fermentation9121007</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
