Probiotic Properties Study of Bacterial Concentrate
https://doi.org/10.36107/spfp.2022.311
Abstract
Background: In modern conditions, it is necessary to maintain and restore the microecological status of a person with the help of probiotics. Factors such as environmental degradation, stress, the use of preservatives in food, diseases are the causes of dysbacteriosis. Probiotic bacteria have a multilevel effect on the human body: restoration of normal intestinal microflora, synthesis of biologically active substances, strengthening of the intestinal barrier, suppression of the growth of pathogenic and opportunistic bacteria. The use of multicomponent probiotics in the treatment of dysbiosis provides a complex effect of various metabolites on the intestinal microbiota. Multi-strain microbial communities have a higher adaptive potential compared to single-species preparations. However, the creation of multi-strain microbial communities requires careful selection for their biocompatibility.
Purpose: to investigate the probiotic properties of the bacterial concentrate of the microbial consortium (MC), obtained by long-term autoselection of the microflora of kefir fungal starter at low pH values.
Materials and methods: A frozen bacterial concentrate of a microbial consortium obtained by growing biomass on curd whey with the addition of growth components was studied. For comparative analysis used the original inoculum - microbial consortium.
Results: As a result of autoselection, a symbiotic community of microorganisms is formed, consisting of thermophilic and mesophilic lactobacilli, lactose-fermenting and non-fermenting yeasts, and acetobacteria. The resulting MC is identical in composition to the natural population of kurunga and koumiss microorganisms.
Conclusion: The article presents data on the influence of various factors simulating the conditions of the human gastrointestinal tract on the survival of the MK microflora and bacterial concentrate. Data on adhesive and antagonistic activity are presented.
References
1. Aleshkin A.V. Clinical and laboratory evaluation of the effectiveness of the drug "BifidumMulti" in the dispensary treatment of children with cancer./ Aleshkin A.V., Amerkhanova A.M., Voropaeva E.A., Efimova O.G., Zeitlin G.Ya., Konovalova M.V.// Medical almanac. 2010. No. 1(10). pp.193-195
2. Begunova A.V. Lactic acid and propionic acid bacteria for obtaining functional products with bifidogenic and hypotensive properties / Begunova A.V., Rozhkova I.V., Zvereva E.A., Glazunova O.A., Fedorova T.V.// Applied biochemistry and microbiology. 2019. Volume 55, No. 6, С566-577
3. Brilis, V.I. Technique for studying the adhesive process of microorganisms / VI Brilis // Laboratory business. 1986. No. 4. S. 210–214.
4. Bukachakova L.Ch. Study of the physicochemical and microbiological parameters of the starter culture of the Altai fermented milk drink chegen [Electronic issue] / Bukachakova L.Ch., Arsenyeva T.P. URL: https://cyberleninka.ru/article/n/issledovanie-fiziko-himicheskih-i-mikrobiologicheskih-pokazateley-zakvaski-altayskogo-kislomolochnogo-napitka-chegen/pdf
5. Ermolaeva A.N. Study of cultures of lactic acid microorganisms isolated from koumiss from various regions of Northern Kazakhstan / Ermolaeva A.N., [et al.]// Biotechnology, theory and practice -2012. -Issue 3 - P. 87-90
6. Zandanova T.N. et al. Research on the possibility of obtaining an associative starter for kurunga / Zandanova T.N., Losorova Yu.E., Myryanova T.P.// Bulletin of the KrasGAU 2020.
7. Zandanova T.N., Gogoleva P.A. Selection of a nutrient medium for obtaining a bacterial concentrate of a microbial consortium / Vestnik KrasGAU. 2018. No. 5 P.67-72.
8. Ivashkina N.Yu., Botkina S.G. The original domestic probiotic acipol: molecular biological and metabolic characteristics / RJGGK. original research. 2009. No. 2. pp.58-64
9. Krieger O.V. Topical issues of creating functional drinks with antibiotic properties// Topical issues of the beverage industry. - 2017. - Issue 1 - P.62-64
10. Krumlikov V.Yu. Selection of stabilization parameters (freezing and drying) of a symbiotic consortium in order to obtain a direct introduction starter / Krumlikov V.Yu., Ostroumov L.A., Sukhikh S.A., Kriger O.V.// ISSN 2074-9414 Food Processing Technigues and technology. 2016.Vol.41.N3 - P.25-29 URL: https://cyberleninka.ru/article/n/podbor-parametrov-stabilizatsii-zamorazhivanie-i-sushka-simbioticheskogo-konsortsiuma-s-tselyu-polucheniya-zakvaski -pryamogo/pdf
11. Mazankova L.N., Lykova E.A. Probiotics: drug characteristics and choice in pediatric practice. Children's infections, 2004, 1-S.18-24
12. Mayagsky A.N. Dysbacteriosis: illusions and reality // Pediatrics. -2000.-Iss.4.-P.80-88.
13. Novik, G.I. Biological activity of probiont microorganisms / G.I. Novik, [et al.] // Prikl. biochem. and microbiol. - 2006 - T.42, No. 2. - pp. 187-194
14. Stoyanova L.G. Isolation and identification of lactic acid bacteria Lactococcus lactis subsp. lactis with antimicrobial activity / Izvestiya TSKhA, issue 5. 2017. pp. 41-65. URL: https://cyberleninka.ru/article/n/vydelenie-i-identifikatsiya-molochnokislyh-bakteriy-lactococcus-lactis-subsp-lactis-s-antimikrobnym-deystviem/pdf
15. Khamnaeva N.I., Olmoeva V.D., Namsaraeva Z.M. The study of the antagonistic activity of the combined inoculum based on the microbial association of kefir fungi / Khamnaeva N.I., [et al.]//Bulletin of the ESSUTU. ― 2016. ― No. 4 (61) ― P. 84-88
16. Cagno, R. Assessing the proteolytic and lipolytic activities of single strains of mesophuic lactobacilli as adjunct cultures usinga Caciotta cheese model system / R. Cagno, [et all] // Int.Dairy J. -2006. –V.16. –P. 119-130.
17. Casadesús J., Low D. Epigenetic gene regulation in the bacterial world. Microbiol. Mol. Biol. Rev. 2006, 70 (3): 830-856.
18. Qin J. et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature. 2010, 4; 464 (7285) Р.59-65.
19. Stark P.L. The microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life /Stark P.L., Lee A //J.Med.Microbial―1982―№2―P.189-203
20. Рaschos K., Allday M.J. Epigenetic reprogramming of host genes in viral and microbial pathogenesis. Trends Microbiol. 2010, 18 (10): 439-447.
Review
For citations:
Zandanova T.N. Probiotic Properties Study of Bacterial Concentrate. Storage and Processing of Farm Products. 2022;(3). (In Russ.) https://doi.org/10.36107/spfp.2022.311
Views:
339
Email this article 