Preview

Storage and Processing of Farm Products

Advanced search

Creation of Functional Fermented Milk Products Based on New Strains of Lactic Acid Bacteria with High Organoleptic Characteristics

https://doi.org/10.36107/spfp.2023.369

Abstract

Introduction: The article deals with modern problems of functional nutrition and products that contribute to the prevention of dysbiotic conditions of the body. The current direction is to expand the functional properties of fermented milk products by screening new strains of starter cultures, optimizing technological techniques, including the selection of effective carrier matrices for probiotic microorganisms, and preserving their viability. At the same time, the levels of colony-forming units of lactic acid bacteria necessary for preventive action are quite rare in food products presented on the Russian market, it is also necessary to pay attention to the organoleptic characteristics of newly created products due to the high titers of microorganisms forming lactic acid and other pH-acidifying metabolites.


Purpose: The aim of the study is to create samples of a biological product with functional properties based on lactic acid bacteria with high organoleptic characteristics that meet the requirements of TR CU 033/2013, and the level of viable microbial cells of at least 1011 CFU/cm3.


Materials and Methods: The object of the study were the above samples of fermented milk products.At the same time, first of all, probiotic strains from the collection of ROSBIOTECH were screened, samples of fermented milk probiotic product were made on the basis of the selected strains according to the standard procedure using pasteurized milk with a mass fraction of fat — 1.5 % as a carrier matrix, then the organoleptic properties of the samples obtained and the level of cell viability were evaluated probiotic microorganisms.


Results: The number of colony-forming units of probiotic microorganisms in the prepared beverages ranged from 2.8 × 1012 to 1.5 × 1013 CFU/cm3,which is high values and contributes to an increase in the preventive effect on the macroorganism. Samples 2407 (b), 6769, 2523 had the best organoleptic characteristics,that is, in 2 variants out of 3, a strain of the species Lactococcus lactis was used, while all samples met the requirements of TR CU 033/2013.


Conclusions: Samples of functional probiotic drinks based on 5 strains of lactic acid bacteria, approved and offered for use in the food industry according to sanitary norms and rules 2.3.2.1078-01, were obtained. «Hygienic requirements for the safety and nutritional value of food products», with a level of viable cells not lower than 1012 CFU/cm3, which allows us to consider samples as prototypes of functional food products for use in real practice.

About the Authors

Marya S. Kanochkina
Limited liability company "Microbial nutrients immunocorrectors" Russian Biotechnological University (BIOTECH University)
Russian Federation


Marya G. Shipareva
Russian Biotechnological University (BIOTECH University)
Russian Federation


Anastasia S. Bilyalova
Federal State Budgetary Institution of Science "Federal Research Center for Nutrition, Biotechnology and Food Safety"
Russian Federation


Nikolay B. Smirnov
Russian Biotechnological University (BIOTECH University)
Russian Federation


References

1. Abraham B. P., Quigley E. M. (2017) Probiotics in Inflammatory Bowel Disease, Gastroenterology clinics of North America, 2017 Dec, 46(4):769-782, doi:10.1016/j.gtc.2017.08.003.

2. Battistini C, Gullón B, Ichimura ES, Gomes AMP, Ribeiro EP, Kunigk L, Moreira JUV, Jurkiewicz C. (2018) Development and characterization of an innovative synbiotic fermented beverage based on vegetable soybean, Brazilian Journal of Microbiology 2018 Apr-Jun, 49(2):303-309, doi:10.1016/j.bjm.2017.08.006.

3. Cavanagh D., Fitzgerald G.F., McAuliffe O. (2015) From field to fermentation: the origins of Lactococcus lactis and its domestication to the dairy environment, Food Microbiology, 2015 May, 47:45-61, doi:10.1016/j.fm.2014.11.001.

4. Centurion F., Basit A.W., Liu J., Gaisford S., Rahim M.A., Kalantar-Zadeh K. (2021) Nanoencapsulation for Probiotic Delivery, ACS Nano: American Chemical Society Publications 2021 Dec, 15(12):18653-18660. doi:10.1021/acsnano.1c09951.

5. Cesselin B., Garrigues C., Pedersen M.B., Roussel C., Gruss A., Gaudu P. (2018) Task Distribution between Acetate and Acetoin Pathways To Prolong Growth in Lactococcus lactis under Respiration Conditions Applied and Environmental Microbiology 2018 Aug ,84(18):e01005-18, doi:10.1128/AEM.01005-18.

6. Duskova M., Sedo O., Ksicova K., Zdrahal Zb., Karpiskova R. (2012) Identification of lactobacilli isolated from food by genotypic methods and MALDI–TOF MS, Int. J. Food Microbiol, 2012, Vol.159, P.107–114.

7. Flach J., van der Waal M.B., van den Nieuwboer M., Claassen E., Larsen O.A. (2018) The underexposed role of food matrices in probiotic products: Reviewing the relationship between carrier matrices and product parameters, Critical Reviews in Food Science and Nutrition 2018, 58(15):2570-2584, doi:10.1080/10408398.2017.1334624.

8. Gao Y., Wang X., Xue C., Wei Z.Crit (2021) Latest developments in food-grade delivery systems for probiotics: A systematic review, Critical Reviews in Food Science and Nutrition 2021 Nov 8:1-18, doi:0.1080/10408398.2021.2001640.

9. Gaudu P., Yamamoto Y., Jensen P.R., Hammer K., Lechardeur D., Gruss A. (2019) Genetics of Lactococci, Microbiol Spectr. 2019 Jul, 7(4), doi:10.1128/microbiolspec.GPP3-0035-2018

10. Haghshenas B., Nami Y., Haghshenas M., Abdullah N., Rosli R., Radiah D., Khosroushahi A.Y. (2015) Bioactivity characterization of Lactobacillus strains isolated from dairy products, Microbiology open, 2015 Oct, 4(5):803-13, doi:10.1002/mbo3.280.

11. Jalc D., Varadyova Z., Mihalikova K., Laukova A. (2015) Nutritional status of corn silage after probiotic conservation, Journal of Applied Animal Research, 2015, Vol.43, N.2, P.141–146.

12. Jiang T., Li H., Han G. G., Singh B., Kang S., Bok J., Kim D., Hong Z., Choi Y., Cho C. (2017) Oral Delivery of Probiotics in Poultry Using pH-Sensitive Tablets, Journal of Microbiology and Biotechnology 2017 Apr, 27(4):739-746. doi:10.4014/jmb.1606.06071.

13. Jones J.J., Stump M.J., Fleming R.C., Lay J.O., Wilkins C.L. (2003) Investigation of MALDI–TOF and FT–MS techniques for analysis of Escherichia coli whole cells, Science Journal of Analytical Chemistry 2003, V.75, P.1340–1347.

14. Kleerebezem M., Bachmann H., van Pelt-KleinJan E., Douwenga S., Smid E.J., Teusink B., van Mastrigt O. (2020) Lifestyle, metabolism and environmental adaptation in Lactococcus lactis FEMS Microbiology Reviews 2020 Nov 24, 44(6):804-820, doi: 10.1093/femsre/fuaa033.

15. Mozaffarian D. (2019) Dairy Foods, Obesity, and Metabolic Health: The Role of the Food Matrix Compared with Single Nutrients, Advances in Nutrition 2019 Sep 1, 10(5):917S-923S, doi:10.1093/advances/nmz053

16. Nagaoka S. (2019) Yogurt Production, Methods in molecular biology, 2019, 1887:45-54, doi:10.1007/978-1-4939-8907-2_5.

17. Ouzari H., Cherif A., Mora D.J. (2002) Autolytic phenotype of Lactococcus lactis strains isolated from traditional Tunisian dairy products, Journal of Applied Microbiology 2002, 92(5):812-20, doi:10.1046/j.1365-2672.2002.01585.x.

18. Ringel-Kulka T., Palsson O. S., Maier D., Carroll I., Galanko J. A., Leyer G., Ringel Y. (2011) Probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 versus placebo for the symptoms of bloating in patients with functional bowel disorders: a double-blind study, Journal of Clinical Gastroenterology 2011 Jul, 45(6):518-25, doi:10.1097/MCG.0b013e31820ca4d6.

19. Sanders M.E., Marco M.L. (2010) Food formats for effective delivery of probiotics, Annual review of food science and technology 2010,1:65-85, doi:10.1146/annurev.food.080708.100743.

20. Song A.A., In LLA, Lim SHE, Rahim R.A. (2017) A review on Lactococcus lactis: from food to factory, Microbial Cell Factories 2017 Apr, 16(1):55, doi: 10.1186/s12934-017-0669-x.

21. Trush E.A., Poluektova E.A., Beniashvilli A.G., Shifrin O.S., Poluektov Y.M., Ivashkin V.T. (2020) The Evolution of Human Probiotics: Challenges and Prospects, Probiotics Antimicrobial Proteins, 2020 Dec, 12(4):1291-1299, doi:10.1007/s12602-019-09628-4.

22. Venema K., Verhoeven J., Beckman C., Keller D. (2020) Survival of a probiotic-containing product using capsule-within-capsule technology in an in vitro model of the stomach and small intestine (TIM-1), 2020 Aug, 11(4):403-409. doi:10.3920/BM2019.0209.

23. Wang J, Zhong Z, Zhang W, Bao Q, Wei A, Meng H, Zhang H. (2012) Comparative analysis of the gene expression profile of probiotic Lactobacillus casei Zhang with and without fermented milk as a vehicle during transit in a simulated gastrointestinal tract, Research in Microbiology 2012, Jun, 163(5):357-65, doi:10.1016/j.resmic.2012.04.002.

24. Wang Y., Wu J., Lv M., Shao Z., Hungwe M., Wang J., Bai X., Xie J., Wang Y., Geng W. (2021) Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry, Frontiers in Bioengineering and Biotechnology, 2021 May 12;9:612285, doi:10.3389/fbioe.2021.612285.

25. Zommiti M., Chikindas M.L., Ferchichi M. (2020) Probiotics-Live Biotherapeutics: a Story of Success, Limitations, and Future Prospects-Not Only for Humans, Probiotics Antimicrobial Proteins, 2020 Sep, 12(3):1266-1289, doi:10.1007/s12602-019-09570-5.

26. Ильина Е.Н. (2009) Прямое МАЛДИ масс-спектрометрическое профилирование бактериальных белков для индикации и характеристики патогенов, Acta naturae, 2009, № 1, С.115–121.

27. Квасников Е.И., Нестеренко О.А. (1966) Некоторые вопросы систематики молочнокислых бактерий, Успехи микробиологии – 1966. – Т.3. – С.3–23.

28. Квасников Е.И., Нестеренко О.А. (1975) Молочнокислые бактерии и пути их использования, М.: Изд-во Наука, 1975. – 384 с.


Review

For citations:


Kanochkina M.S., Shipareva M.G., Bilyalova A.S., Smirnov N.B. Creation of Functional Fermented Milk Products Based on New Strains of Lactic Acid Bacteria with High Organoleptic Characteristics. Storage and Processing of Farm Products. 2023;(2):176-186. (In Russ.) https://doi.org/10.36107/spfp.2023.369

Views: 338


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2072-9669 (Print)
ISSN 2658-767X (Online)