Development of an Active Starch-Based Packaging Material Using Clove Oil Extract as an Antimicrobial Additive
https://doi.org/10.36107/spfp.2023.4.474
Abstract
Introduction: With increasing pressure on the environment, there is an increasing need to eliminate the use of chemicals as antimicrobial agents in the packaging industry, which raises the need to find innovative natural solutions to replace these substances. In this study, starch-based packaging materials with the introduction of a natural antimicrobial additive were developed as a potential answer to this problem. The use of new environmentally friendly materials can extend the shelf life of food products, ensuring preservation and safety.
Purpose: Development of active packaging film from biopolymer with introduction of clove essential oil into it, determination of antimicrobial properties of the obtained polymeric material.
Materials and Methods: In this work, starch-based film was prepared by mechanical mixing of initial reagents with the introduction of essential oil of different concentrations. Then antimicrobial properties of polymeric packaging material were determined by disk-diffusion method in accordance with MUK 4.2.1890-04 in relation to (Bacillus subtilis), (Escherichia coli), (Candida albicans) and (Aspergillus niger), also an experiment was conducted to determine the ability of films with essential oil to reduce the amount of CMAFANM on the surface of sausage products during their storage.
Results: It was found that samples of packaging film based on starch with clove essential oil have antimicrobial activity against Escherichia coli, Bacillus subtilis, Candida albicans, Aspergillus niger and reduce the number of microorganisms colonies on the surface of the food product.
Conclusions: The developed material has a high potential for use in the food industry, as it allows to improve the quality and safety of products, in particular sausage products, as well as to reduce the amount of plastic synthetic packaging materials that have a negative impact on the environment. The obtained material with antimicrobial properties can be used for packaging of various food products.
About the Authors
Ali AlkhairRussian Federation
Ekaterina Alexandrovna Shchukina
Russian Federation
Marina Ivanovna Gubanova
Russian Federation
Irina Anatolyevna Kirsh
Russian Federation
Alexandra Maksimovna Ermilova
Russian Federation
Isabella S. Tveritnikova
Russian Federation
References
1. A STUDY ON THE CHEMICAL PROPERTIES OF EUGENOL AND EUGENOL ACETATE, CLOVE ESSENTIAL OILS https://www.researchgate.net/publication/308902393
2. Antibacterial and immunostimulatory effect of essential oils. https://www.researchgate.net/publication/285651532_Antibacterial_and_immunostimulatory_effect_of_essential_oils
3. Atar´es, L., & Chiralt, A. (2016). Essential oils as additives in biodegradable films and coatings for active food packaging. Trends in Food Science and Technology, 48, 51–62.
4. Basch E, Gasparyan A, Giese N, Hashmi S, Miranda M, Sollars D, Seamon E, Tanguay-Colucci S, Ulbricht C, Varghese M, Vora M, Weissner W. Clove (Eugenia aromatica) and clove oil (eugenol). Natural standard monograph (www.naturalstandard.com) copyright© 2008. J Diet Suppl. 2008;5:117–146.
5. Batiha G.E., Alkazmi L.M., Wasef L.G., Beshbishy A.M., Nadwa E.H., Rashwan E.K. Syzygium aromaticum L. (Myrtaceae): Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities. Biomolecules. 2020;10:202. doi: 10.3390/biom10020202.
6. Design of multifunctional food packaging films based on carboxymethyl chitosan/polyvinyl alcohol crosslinked network by using citric acid as crosslinker. Lishan Wen,Yuntong Liang,Zhenhao Lin,Donghong Xie,Zhongjie Zheng,Chuanhui Xu,Baofeng Lin. Polymer. Volume 230, 16 September 2021, 124048. https://doi.org/10.1016/j.polymer.2021.124048.
7. Eugenol_and_linalool_Comparison_of_their_antibacterial_and_antifungal_activities. https://www.researchgate.net/publication/311365020_. DOI:10.5897/AJMR2016.8283
8. Fangjun L., Zhijia Y. Tumor suppressive roles of eugenol in human lung cancer cells. Thorac. Cancer. 2018;9:25–29. doi: 10.1111/1759-7714.12508.
9. G. Ebenezer Jeyakumar, Rubina Lawrence. Mechanisms of bactericidal action of Eugenol against Escherichia coli. Journal of Herbal Medicine. Volume 26, April 2021, 100406. Https://doi.org/10.1016/j.hermed.2020.100406.
10. January 2011International Review of Allergology and Clinical Immunology. Monika Sienkiewicz, P. Denys, E. Kowalcz https://www.researchgate.net/publication/285651532_Antibacterial_and_immunostimulatory_effect_of_essential_oils.
11. Kamatou GP, Vermaak I, Viljoen AM. Eugenol; from the remote Maluku Islands to the international market place: a review of a remarkable and versatile molecule. Molecules. 2012;17:6953–6981.
12. L. NuñezM. D' Aquino. Microbicide activity of clove essential oil (Eugenia caryophyllata). Brazilian Journal of Microbiology. 2012 Oct-Dec; 43(4): 1255–1260. doi: 10.1590/S1517-83822012000400003.
13. Magdalena Ulanowska and Beata Olas. Biological Properties and Prospects for the Application of Eugenol—A Review. International Journal of Molecular Sciences. 2021 Apr; 22(7): 3671.doi: 10.3390/ijms22073671.
14. Matan N., Rimkeeree H., Mawson A.J., Chompreeda P., Haruthaithanasan V., Parker M. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. J. Food Microbiol. 2006;107:180–185.
15. Md. Ashrafudoulla, Md. Furkanur Rahaman Mizan, Si Hong Park & SangDo Ha Current and future perspectives for controlling Vibrio biofilms in the seafood industry: a comprehensive review. Critical Reviews in Food Science and Nutrition 2020, DOI: 10.1080/10408398.2020.1767031.
16. Ojagh, S. M., Rezaei, M., Razavi, S. H., & Hosseini, S. M. H. (2010). Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food Chemistry, 122(1), 161–166.
17. Pires, C., Ramos, C., Teixeira, B., Batista, I., Nunes, M. L., & Marques, A. (2013). Hake proteins edible films incorporated with essential oils: Physical, mechanical, antioxidant and antibacterial properties. Food Hydrocolloids, 30(1), 224–231.
18. Sadekuzzaman, M., S. Yang, M. F. R. Mizan, and S. D. Ha. 2015. Current and recent advanced strategies for combating biofilms. Comprehensive Reviews in Food Science and Food Safety 14 (4): 491–509. doi: 10.1111/1541-4337.12144.
19. Shubham Sharma, Sandra Barkauskaite, Amit K. Jaiswal, Swarna Jaiswal. Essential oils as additives in active food packaging. Food Chemistry. Volume 343, 1 May 2021, 128403. https://doi.org/10.1016/j.foodchem.2020.128403.
20. Tajkarimi M.M., Ibrahim S.A., Cliver D.O. Antimicrobial herb and spice compounds in food. Food Control. 2010;21:1199–2121.
21. Turk J Pharm Sci. 2017 Aug; 14(2): 201–206. Pharmacological and Toxicological Properties of Eugenol. Solmaz MOHAMMADI NEJAD, Hilal ÖZGÜNEŞ, and Nursen BAŞARAN. Pharmacological and Toxicological Properties of Eugenol. doi: 10.4274/tjps.62207.
22. Versatile and Synergistic Potential of Eugenol: A Review. Mamilla R Charan Raja, Varsha Srinivasan, Sharmila Selvaraj and Santanu Kar Mahapatra*. https://www.walshmedicalmedia.com/open-access/versatile-and-synergistic-potential-of-eugenol-a-review-2153-2435-1000367.pdf. DOI: 10.4172/2153-2435.1000367
23. Walsh S.E., Maillard J.-Y., Russell A.D., Catrenich C.E., Charbonneau D.L., Bartola R.G. Activity and mechanisms of action of selected biocidal agents on Gram-positive and -negative bacteria. J. Appl. Microbiol. 2003;94:240–247.
24. Wangwei, L., Rui, C., Bifen, Z., Yuyue, Q., Guiguang, C., Lin, L., & Minglong, Y. (2021). Influence of clove essential oil immobilized in mesoporous silica nanoparticles on the functional properties of poly (lactic acid) biocomposite food packaging film. Journal of Materials Research and Technology, 11, 1152-1161. https://doi.org/10.1016/j.jmrt.2021.01.098.
25. Shubham, S., Sandra, B., Brendan, D., Amit K. J., & Swarna, J. (2020). Characterization and Antimicrobial Activity of Biodegradable Active Packaging Enriched with Clove and Thyme Essential Oil for Food Packaging Application. Foods, 9, 1-16. https://doi.org/10.3390/foods9081117.
26. Silva, I. D. L., Andrade, M. F., Caetano, V. F., Hallwass, F., Brito, A. M. S. S., & Vinhas, G. M. (2020). Development of active PHB/PEG antimicrobial films incorporating clove essential oil. Polímeros: Ciência e Tecnologia, 30(2), e2020021. https://doi.org/10.1590/0104-1428.09319.
Supplementary files
Review
For citations:
Alkhair A., Shchukina E.A., Gubanova M.I., Kirsh I.A., Ermilova A.M., Tveritnikova I.S. Development of an Active Starch-Based Packaging Material Using Clove Oil Extract as an Antimicrobial Additive. Storage and Processing of Farm Products. 2023;(4):16-31. (In Russ.) https://doi.org/10.36107/spfp.2023.4.474