Screening of Bacteriocin-Producing Strains of Lactic Acid Bacteria to Create a Drug with Antimicrobial Properties

Abstract

The problem of finding and studying the properties of new strains-producers of bacteriocins, promising for the subsequent creation of drugs and technological AIDS for use in the food industry, as well as the selection of optimal cultivation conditions for the synthesis of bacteriocins is relevant. Lactic acid bacteria produce a wide range of bacteriocins that can delay growth or completely destroy the development of microorganisms that cause damage to fruits and vegetables. Screening of lactic acid bacteria by acid-forming activity and accumulation of bacteriocin-like substances was carried out. Studied the qualitative composition of the metabolites formed by the studied strains in MRS medium, showed the characteristic bacteriocins: acidosis, acidophilus, plantaricin, salivaricin, nisin, Lactococcus. As the most promising selected culture 1660 Lactobacillus acidophilus, Lactobacillus plantarum 578/26, producer Lactococcus lactis subsp. lactis 1500/12, synthesizing respectively 8.25; 7.91 and 4.27 mg/dm³ bacteriocins. It is established that metabolites of the selected cultures possess the expressed antimicrobial properties, especially concerning bacterial forms of microorganisms, and also oppress growth of the causative agent of spoilage of vegetables and fruits Nocardia asteroids. In further studies, it was found that with the joint cultivation of the selected strains, the number of bacteriocins can be increased by optimizing the nutrient medium and selecting the cultivation conditions. When the consortium is jointly cultivated, L-lactic acid, soluble proteins, catalase-peroxidase and lactate dehydrogenase complexes accumulate in the culture liquid. In addition, discovered zitohromoksidasy systems, as well as b vitamins Created by a consortium of selected strains of lactic acid bacteria to create drugs with antimicrobial properties.