Evaluation of the Immunogenicity and Antigen Content of A. Ovis of an Experimental Sample of the Adsorbed Inactivated Culture-Cell Vaccine Against Sheep Anaplasmosis
https://doi.org/10.36107/spfp.2020.376
Abstract
Sheep anaplasmosis is a blood-parasitic vector-borne disease caused by the endoglobular intra-erythrocyte parasite Anaplasma. The main signs of anaplasmosis are anemia, emaciation, loss of productivity and violation of the reproductive function of animals. Vaccination with an adsorbed inactivated culture-cell vaccine is promising for the prevention of sheep anaplasmosis. The conducted studies made it possible to evaluate the immunogenicity of an experimental sample of a vaccine made using preparations of mixed cultures of leukocytes from immunized animals, and the content of the A. ovis antigen using PCR. Comparison of experimental samples of culture-cell vaccine before and after storage in terms of immunogenicity and A. ovis antigen content with extrapolation of the degree of spontaneous inactivation of the vaccine with a real storage duration of 1 year showed that the above indicators did not deteriorate.
About the Authors
R. V. Glushakov
LLC “EuroPack”
Russian Federation
Russian Federation
Roman V. Glushakov
115088, Moscow, 3rd Ugreshsky proezd, house 8, building 9
I. A. Vasilenko
LLC „EuroPack“
Russian Federation
Russian Federation
Ivan A. Vasilenko
115088, Moscow, 3rd Ugreshsky proezd, house 8, building 9
A. A. Donskikh
LLC “EuroPack”
Russian Federation
Russian Federation
Alexey A. Donskikh
115088, Moscow, 3rd Ugreshsky proezd, house 8, building 9
M. P. Shchetinin
FSBEI HE “MGUPP”
Russian Federation
Russian Federation
Mikhail P. Shchetinin
125371, Moscow, Volokolamskoe highway, 11
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Review
For citations:
Glushakov R.V., Vasilenko I.A., Donskikh A.A., Shchetinin M.P. Evaluation of the Immunogenicity and Antigen Content of A. Ovis of an Experimental Sample of the Adsorbed Inactivated Culture-Cell Vaccine Against Sheep Anaplasmosis. Storage and Processing of Farm Products. 2020;(3):82-101. (In Russ.) https://doi.org/10.36107/spfp.2020.376
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