A Study of the Effectiveness of an Enzyme Preparation Obtained from a New Mutant Trichoderma Reesei Strain in the Cereal Mixtures Hydrolysis

Abstract

Most of the farm animals feeds in Russia are produced on the basis of grain raw materials with a high content of non-starch polysaccharides, which have an adverse effect on the organism of monogastric animals and poultry. Therefore, the bulk of feed enzymes comprise endoglucanases and xylanases capable of destroying the anti-nutritional carbohydrate components of the grain raw materials - cellulose and arabinoxylan, respectively, with the formation of digestible sugars and prebiotic oligosaccharides. In order to increase the economic indicators of the enzyme preparations production and the effectiveness of their use in the feed industry, studies are being conducted to obtain high-producing strains. In VNIIPBT using UV-mutagenesis T. reesei 28-U-12 strain was obtained with increased xylanase and endoglucanase activity. Samples of dry concentrated preparations based on the initial and mutant strains were produced. The purpose of the work was to determine the effectiveness of the preparations in processing of rye meal and wheat / barley meal mixture. The hydrolysis of the substrates was carried out in shaking flasks at 220 rpm at 40 °C. Substrate concentration was 50 mg/ml; preparations were added at dosages of 1, 3, and 5 mg per 1 g of raw materials. Soluble protein and reducing sugars content was determined in supernatants of the obtained hydrolysates. The use of endoglucanase and xylanase preparations increased the yield of reducing sugars by 2-3 times on all types of raw materials. Increased activity of key enzymes in the mutant strain allowed to reduce the consumption of the preparation by 35-40 % compared to the preparation from the original strain. The use of the preparation on the basis of a mutant strain also contributed to an increase in the yield of soluble protein by 10-15 %.